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positive control atcc 49619  (ATCC)


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    ATCC positive control atcc 49619
    Positive Control Atcc 49619, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3001 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/positive+control+atcc+49619/Streptococcus+pneumoniae%3B+262/pmc12756702-200-3-5
    Average 99 stars, based on 3001 article reviews
    positive control atcc 49619 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Positive Control:

    Article Title: Serotype diversity and risk factors for pneumococcal carriage among healthy children in Klang Valley, Malaysia: A pre-vaccination cross-sectional study
    Article Snippet: Amplification was performed using the Mastercycler® nexus X2 (Eppendorf, Germany) under the following cycling conditions: initial denaturation at 95°C for 4 minutes; followed by 35 cycles of denaturation at 95°C for 45 seconds, annealing at 54°C for 45 seconds, and extension at 65°C for 2 minutes and 30 seconds. .. All runs included positive control ATCC 49619 and negative control ATCC 25922 for quality assurance. .. Following amplification, 4 μl of each PCR product was loaded onto a 2.0% agarose gel alongside a 100 bp DNA ladder (GeneDireX, USA).

    Negative Control:

    Article Title: Serotype diversity and risk factors for pneumococcal carriage among healthy children in Klang Valley, Malaysia: A pre-vaccination cross-sectional study
    Article Snippet: Amplification was performed using the Mastercycler® nexus X2 (Eppendorf, Germany) under the following cycling conditions: initial denaturation at 95°C for 4 minutes; followed by 35 cycles of denaturation at 95°C for 45 seconds, annealing at 54°C for 45 seconds, and extension at 65°C for 2 minutes and 30 seconds. .. All runs included positive control ATCC 49619 and negative control ATCC 25922 for quality assurance. .. Following amplification, 4 μl of each PCR product was loaded onto a 2.0% agarose gel alongside a 100 bp DNA ladder (GeneDireX, USA).



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    Agarose gels containing representative amplicons. (A) Universal primers RW01 and DG74. Lanes 1 and 9, 100-bp ladder; lane 2, negative control for the extraction process; lane 3, negative control for <t>PCR;</t> lane 4, positive control for PCR (Escherichia <t>coli</t> <t>ATCC</t> 25922 DNA); lanes 5 to 7, three PCR-positive MEE samples, respectively (370-bp band); lane 8, a PCR-negative MEE sample. (B) Haemophilus-specific primer-probe pair RDR125 and DG74. Lanes 1 and 9, 50-bp ladder; lane 2, empty; lane 3, negative control for PCR; lane 4, positive control for PCR (H. influenzae ATCC 49766 DNA); lane 5, PCR-positive MEE sample (124-bp band); lanes 6 and 7, two PCR-negative MEE samples, respectively; lane 8, empty. (C) Haemophilus-specific F1-R1 primer pair. Lanes 1 and 9, 100-bp ladder; lane 2, empty; lane 3, negative control for PCR; lane 4, positive control for PCR (H. influenzae ATCC 49766 DNA); lane 5, PCR-positive MEE sample (351-bp band); lanes 6 and 7, two PCR-negative MEE samples, respectively; lane 8, empty.
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    Image Search Results


    Agarose gels containing representative amplicons. (A) Universal primers RW01 and DG74. Lanes 1 and 9, 100-bp ladder; lane 2, negative control for the extraction process; lane 3, negative control for PCR; lane 4, positive control for PCR (Escherichia coli ATCC 25922 DNA); lanes 5 to 7, three PCR-positive MEE samples, respectively (370-bp band); lane 8, a PCR-negative MEE sample. (B) Haemophilus-specific primer-probe pair RDR125 and DG74. Lanes 1 and 9, 50-bp ladder; lane 2, empty; lane 3, negative control for PCR; lane 4, positive control for PCR (H. influenzae ATCC 49766 DNA); lane 5, PCR-positive MEE sample (124-bp band); lanes 6 and 7, two PCR-negative MEE samples, respectively; lane 8, empty. (C) Haemophilus-specific F1-R1 primer pair. Lanes 1 and 9, 100-bp ladder; lane 2, empty; lane 3, negative control for PCR; lane 4, positive control for PCR (H. influenzae ATCC 49766 DNA); lane 5, PCR-positive MEE sample (351-bp band); lanes 6 and 7, two PCR-negative MEE samples, respectively; lane 8, empty.

    Journal:

    Article Title: Two-Step PCR-Based Assay for Identification of Bacterial Etiology of Otitis Media with Effusion in Infected Lebanese Children

    doi:

    Figure Lengend Snippet: Agarose gels containing representative amplicons. (A) Universal primers RW01 and DG74. Lanes 1 and 9, 100-bp ladder; lane 2, negative control for the extraction process; lane 3, negative control for PCR; lane 4, positive control for PCR (Escherichia coli ATCC 25922 DNA); lanes 5 to 7, three PCR-positive MEE samples, respectively (370-bp band); lane 8, a PCR-negative MEE sample. (B) Haemophilus-specific primer-probe pair RDR125 and DG74. Lanes 1 and 9, 50-bp ladder; lane 2, empty; lane 3, negative control for PCR; lane 4, positive control for PCR (H. influenzae ATCC 49766 DNA); lane 5, PCR-positive MEE sample (124-bp band); lanes 6 and 7, two PCR-negative MEE samples, respectively; lane 8, empty. (C) Haemophilus-specific F1-R1 primer pair. Lanes 1 and 9, 100-bp ladder; lane 2, empty; lane 3, negative control for PCR; lane 4, positive control for PCR (H. influenzae ATCC 49766 DNA); lane 5, PCR-positive MEE sample (351-bp band); lanes 6 and 7, two PCR-negative MEE samples, respectively; lane 8, empty.

    Article Snippet: Lanes 1 and 9, 50-bp ladder; lane 2, empty; lane 3, negative control for PCR; lane 4, positive control for PCR ( S. pneumoniae ATCC 49619 DNA); lanes 5 and 7, two PCR-negative MEE samples, respectively; lane 6, PCR-positive MEE sample (105-bp band); lane 8, empty. (C) M. catarrhalis -specific MCAT1 and MCAT2 primer pair.

    Techniques: Negative Control, Extraction, Positive Control

    Agarose gels containing representative amplicons. (A) Universal primers RW01 and DG74. Lanes 1 and 9, 100-bp ladder; lane 2, negative control for the extraction process; lane 3, negative control for PCR; lane 4, positive control for PCR (E. coli ATCC 25922 DNA); lanes 5 to 7, three PCR-positive MEE samples, respectively (370-bp band); lane 8, PCR-negative MEE sample. (B) Streptococcus-specific STR1 and DG74 primer pair. Lanes 1 and 9, 50-bp ladder; lane 2, empty; lane 3, negative control for PCR; lane 4, positive control for PCR (S. pneumoniae ATCC 49619 DNA); lanes 5 and 7, two PCR-negative MEE samples, respectively; lane 6, PCR-positive MEE sample (105-bp band); lane 8, empty. (C) M. catarrhalis-specific MCAT1 and MCAT2 primer pair. Lanes 1 and 9, 50-bp ladder; lane 2, empty; lane 3, negative control for PCR; lane 4, positive control for PCR (M. catarrhalis DNA); lanes 5 and 6, two PCR-negative MEE samples, respectively; lane 7, a PCR-positive MEE sample (140-bp band); lane 8, empty.

    Journal:

    Article Title: Two-Step PCR-Based Assay for Identification of Bacterial Etiology of Otitis Media with Effusion in Infected Lebanese Children

    doi:

    Figure Lengend Snippet: Agarose gels containing representative amplicons. (A) Universal primers RW01 and DG74. Lanes 1 and 9, 100-bp ladder; lane 2, negative control for the extraction process; lane 3, negative control for PCR; lane 4, positive control for PCR (E. coli ATCC 25922 DNA); lanes 5 to 7, three PCR-positive MEE samples, respectively (370-bp band); lane 8, PCR-negative MEE sample. (B) Streptococcus-specific STR1 and DG74 primer pair. Lanes 1 and 9, 50-bp ladder; lane 2, empty; lane 3, negative control for PCR; lane 4, positive control for PCR (S. pneumoniae ATCC 49619 DNA); lanes 5 and 7, two PCR-negative MEE samples, respectively; lane 6, PCR-positive MEE sample (105-bp band); lane 8, empty. (C) M. catarrhalis-specific MCAT1 and MCAT2 primer pair. Lanes 1 and 9, 50-bp ladder; lane 2, empty; lane 3, negative control for PCR; lane 4, positive control for PCR (M. catarrhalis DNA); lanes 5 and 6, two PCR-negative MEE samples, respectively; lane 7, a PCR-positive MEE sample (140-bp band); lane 8, empty.

    Article Snippet: Lanes 1 and 9, 50-bp ladder; lane 2, empty; lane 3, negative control for PCR; lane 4, positive control for PCR ( S. pneumoniae ATCC 49619 DNA); lanes 5 and 7, two PCR-negative MEE samples, respectively; lane 6, PCR-positive MEE sample (105-bp band); lane 8, empty. (C) M. catarrhalis -specific MCAT1 and MCAT2 primer pair.

    Techniques: Negative Control, Extraction, Positive Control